Ciencias Moleculares

Química y Biotecnología de Productos Naturales

Nuestro interés científico se centra fundamentalmente en la biotecnología y química de productos naturales, con una investigación interdisciplinar en la que valoramos la diversidad químico-biológica de especies vegetales y microorganismos endófitos.

Perfil del grupo Química y Biotecnología de Productos Naturales en Digital.CSIC.

Image
pn
Presentación

Con este planteamiento aplicamos técnicas biotecnológicas a la producción de sustancias naturales con actividad plaguicida y de interés farmacológico. La mayor parte de nuestra actividad se desarrolla con especies vegetales endémicas del archipiélago canario y especies protegidas de la flora mediterránea.

Estudio fitoquímico y quimiotaxonómico de la biodiversidad canaria

Productos naturales de hongos endófitos de plantas

Producción sostenible de productos naturales en cultivo de tejidos vegetales in vitro y raíces inducidas genéticamente

Biotransformaciones de productos naturales

TFG: Diversidad y distribución de hongos endófitos de Plocama pendula Ait

Año:2017
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dra. Cristina Jiménez Mariño

Estudiante:

Paula González Delgado

Tipo:Máster y TFG

Estudio de la diversidad endofítica de Persea indica y de su potencial bioplaguicida

Año:2017
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández, Dr. Braulio M. Fraga González, Dra. Azucena González Coloma

Estudiante:

Patricia Bolaños González

Tipo:Tesis doctoral

Valorización y producción biotecnológica de biocidas a partir de dos especies de Artemisia (A. americana y A. assoana)

Año:2017
Director del trabajo:

Dra. Azucena González Coloma, Dra. Carmen Elisa Díaz Hernández, Dra. Mª Fe Andrés Yeves

Estudiante:

Paula Sainz Sotomayor

Tipo:Tesis doctoral

TFG: Diversidad y distribución de hongos endófitos en endemismos canarios

Año:2016
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dra. Cristina Jiménez Mariño

Estudiante:

Mihaela Paris

Tipo:Máster y TFG

Producción y obtimización de bioplaguicidas de Artemisia absinthium y Lavandula luisieri

Año:2016
Director del trabajo:

Dra. Azucena González Coloma, Dra. Carmen Elisa Díaz Hernández, Dra. Mª Fe Andrés Yeves

Estudiante:

Luis Fernando Julio Torres

Tipo:Tesis doctoral

TFG: Biotransformación, aislamiento e identificación de terpenos

Año:2015
Director del trabajo:

Dr. Ricardo Guillermo Álvarez y Dra. Carmen Elisa Díaz Hernández

Estudiante:

Saray Díaz López

Tipo:Máster y TFG

TFG: Biodiversidad de hongos endófitos de especies vegetales y potencial bioplaguicida

Año:2015
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dra. Cristina Jiménez Mariño

Estudiante:

Jennifer Rodríguez Hernández

Tipo:Máster y TFG

Compuestos bioactivos de especies del género Senecio (Asteraceae). Estudio de la relación estructura -actividad

Año:2015
Director del trabajo:

Dr. Matías Reina Artiles, Dra Azucena González Coloma

Estudiante:

Liliana Ruiz Vasquez

Tipo:Tesis doctoral

Procesos biotecnológicos y fitoquímicos de plaguicidas botánicos

Año:2015
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández, Dr. Matías Reina Artiles, Dra. Azucena González Coloma

Estudiante:

Adriana González Portero

Tipo:Tesis doctoral

TFG: Biotransformaciones

Año:2014
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dr. Ricardo Guillermo Álvarez

Estudiante:

Leticia Díaz Gómez

Tipo:Máster y TFG

MASTER: Hongos endófitos como precursores de biodiesel

Año:2012
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dr. Ricardo Guillermo Álvarez

Estudiante:

María Alía Martín

Tipo:Máster y TFG

Transformaciones químicas y microbiológicas de diterpenos

Año:2011
Director del trabajo:

Braulio M. Fraga González, Ricardo Guillermo Álvarez

Estudiante:

M. Victoria González de Vallejo

Tipo:Tesis doctoral

MASTER: Procesos Biotecnológicos en obtención de bioplaguicidas botánicos y fúngicos

Año:2011
Director del trabajo:

Dr. B. M. Fraga González y Dra. Elisa Díaz Hernández

Estudiante:

Patricia Bolaños González

Tipo:Máster y TFG

Agentes antiparasitarios de plantas de la Amazonia Peruana empleadas en medicina tradicional

Año:2010
Director del trabajo:

Dra. Azucena González Coloma, Dr. Matías Reina Artiles

Estudiante:

Olga Claudia Sáenz Bocanegra

Tipo:Tesis doctoral

MASTER: Alcaloides pirrolizidínicos de senecios endémicos del Perú

Año:2009
Director del trabajo:

Dr. Matías Reina Artiles y Dra Azucena González-Coloma

Estudiante:

Liliana Ruiz Vázquez

Tipo:Máster y TFG

DEA: Estudio fitoquímico de Pericallis equinata. Evaluaciòn preliminar de su actividad bioplaguicida

Año:2009
Director del trabajo:

Dra. Carmen Elisa Díaz Hernández y Dr. Matías Reina Artiles

Estudiante:

Adriana González Portero

Tipo:Máster y TFG

Aislamiento, biotransformación y síntesis de diterpenos

Año:2008
Director del trabajo:

Dr. Braulio M. Fraga González, Dr. Ricardo Guillermo Álvarez, Dr. Pedro González García

Estudiante:

Carlo Bressa

Tipo:Tesis doctoral

Estudio fitoquímico de raíces transformadas de endemismos canarios

Año:2008
Director del trabajo:

Dr. Braulio M. Fraga González, Dra. Carmen Elisa Díaz Hernández

Estudiante:

Leonardo J. Amador Díaz

Tipo:Tesis doctoral

Nuevos agentes de control parasitario y vectorial

Año:2008
Director del trabajo:

Dra. Azucena González-Coloma, Dra. Carmen Elisa Díaz Hernández, Dr. Rafael Martínez Díaz

Estudiante:

María Bailén Andrino

Tipo:Tesis doctoral
Image
life

Sustainable and green agri-waste based biopesticides

El objetivo principal de WASTE4GREEN es mitigar los efectos adversos sobre el Medio Ambiente y la Salud Humana de los pesticidas de origen químico que se utilizan...

Investigador principal:

Azucena González Coloma (ICA - CSIC)

Estado:

En Ejecución

Image
MINECO

Desarrollo de bioplaguicidas mediante tecnologías químicas y biotecnológicas

Investigador principal:

Azucena González Coloma

Carmen Elisa Díaz Hernández

Estado:

Finalizado

Proyecto de investigación

Desarrollo de prototipos (insecticida y herbicida) de origen botánico de plantas peruanas para el control de plagas de importancia en agricultura

Investigador principal:

Azucena González Coloma

Estado:

Finalizado

Proyecto de investigación

Development of fungal biopesticides from endophytes isolated from medicinal plants

Investigador principal:

Azucena González Coloma

Nutan Kaushik (The Energy and Resources Institute - TERI)

Estado:

Finalizado

Image
MINECO

Producción biotecnológica de bioplaguicidas: optimización y formulación mediante procesos químicos y tecnologías de fluidos supercríticos

Investigador principal:

Azucena González Coloma

Estado:

Finalizado

Image
Ministerio de Ciencia e Innovación

Bioplaguicidas: Producción y optimización mediante procesos químicos y biotecnológicos

Investigador principal:

Azucena González Coloma

Estado:

Finalizado

Image
Ministerio de Ciencia e Innovación

Bioprospección de hongos endófitos de plantas medicinales para la producción de bioplaguicidas

Investigador principal:

Azucena González Coloma

Nutan Kaushik (The Energy and Resources Institute - TERI)

Estado:

Finalizado

Proyecto de investigación

Pesquisa de Novos Agroquímicos Naturais para o Controlo de Pragas

Investigador principal:

Azucena González Coloma

Cristina Moiteiro (Instituto Nacional de Engenharia, Tecnologia e Inovação - INETI)

Estado:

Finalizado

Image
EUROSTARS

Green crop protectants

Investigador principal:

Azucena González Coloma

Estado:

Finalizado

Image
CIENCIA E INNOVACION

Interactions of phytochemicals with taste and feeding of insects

Investigador principal:

Azucena González Coloma (ICA/CSIC)

Frédéric Marion-Poll (INRA)

Estado:

Finalizado

Noticias/Blog

Publicaciones destacadas

Image
Chemical composition and biological

Chemical composition and biological effects of essential oils from Artemisia absinthium L. cultivated under different environmental conditions

The objective of this study was the valorization of the essential oils from Spanish Artemisia absinthium domesticated plants from Teruel and Sierra Nevada (Spain). These populations were experimentally cultivated in the field and under controlled conditions. The insect antifeedant properties of their essential oils collected yearly from two locations were tested against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi. Additionally we studied their phytotoxic, antifungal and antiparasitic effects. The oils from cultivated A. absinthium were characterized by the presence of cis-epoxyocimene, chrysanthenol, and chrysanthenyl acetate. The variations observed in oil composition were mostly quantitative but also qualitative. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol has been isolated and identified by NMR. Among the oil samples, these rich in cis-epoxyocimene and sesquiterpenes were the most active ones against S. littoralis. (Z)-2,6-Dimethyl-5,7-octadien-2,3-diol showed moderate activity against S. littoralis. The strongest antifeedant effects were found for commercial A. absinthium oil samples rich in thujones and sabinyl acetate. F. oxysporum and F. solani were affected by oils from cultivated A. absinthium and commercial oil samples. Oils from cultivated A. absinthium showed antiparasitic effects against Leishmania infantum and Trypanosoma cruzi with better results than the commercial

Bailén, María; Julio, Luis F.; Díaz, Carmen E.; Sanz Perucha, Jesús; Martínez-Díaz, Rafael A.; Cabrera, Raimundo; Burillo Alquézar, Jesús; González-Coloma, Azucena 

Industrial Crops and Products 49: 102-107 (2013)
Image
Major components of Spanish cultivated Artemisa

Major components of Spanish cultivated Artemisia absinthium populations: Antifeedant, antiparasitic, and antioxidant effects

The objective of this study was the valorization of Spanish Artemisia absinthium populations from Teruel (Aragón) and Sierra Nevada (Granada). These populations were experimentally cultivated in the field and under controlled conditions. Three major components were isolated from a two year-old population obtained from the Teruel population cultivated in Ejea-Zaragoza in 2003, and identified by NMR experiments as the sequiterpene lactone hydroxypelenolide (I) and the flavones artemetin (II) and casticin (III). The I–III content of the plant extracts was analyzed by HPLC-DAD. The insect antifeedant properties of plant extracts from different years and crops were tested against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi. Additionally we studied their antioxidant, phytotoxic and antiparasitic effects.

Gonzalez-Coloma, Azucena; Bailen, Maria; Diaz, Carmen E.; Fraga, Braulio M.; Martínez-Díaz, Rafael; Zuñiga, Gustavo E.; Contreras, Rodrigo A.; Cabrera, Raimundo; Burillo, Jesus

Industrial Crops and Products 37(1): 401-407 (2012)
Image
Supercritical CO2 extraction of Persea

Supercritical CO2 extraction of Persea indica: Effect of extraction parameters, modelling and bioactivity of its extracts

The objective of the work was to optimize the extraction of Persea indica L. bioactive compounds by means of supercritical fluid extraction (SFE) and analyze their insecticidal effects. P. indica L. is one of the dominant species of the Canarian laurel forest, a relict of the Tertiary flora. Different extraction conditions (pressure, plant material particle size, temperature, CO2 flow) and the influence of entrainer were tested and the evolution of the extracted compounds was screened by HPLC–MS. A comparison with conventional techniques such as hydrodistillation (HD) or organic solvent extraction (OSE) was also presented. Particularly, four CO2 densities ranging from 628.61 kg/m3 to 839.81 kg/m3 were studied in the range of 10.0–20.0 MPa and 40–50 °C. The extracts contained insecticidal ryanodanes of great interest, previously described as insecticidal components of P. indica. The insecticidal antifeedant activity of selected extracts was inspected. A model based on mass transfer equations, the Sovová model, was successfully applied to correlate the experimental data.

Martín, L; González-Coloma, A.; Díaz, C. E.; Mainara, A. M.; Urieta, J. S.

The Journal of Supercritical Fluids 57(2): 120-128 (2011)
Image
Nematicidal activity of the hydrolate

Nematicidal activity of the hydrolate byproduct from the semi industrial vapor pressure extraction of domesticated Artemisia absinthium against Meloidogyne javanica

The objective of this study was the characterization of nematicidal activity and valorization of hydrolate obtained as a byproduct of the semi industrial vapor-pressure essential oil extraction of a domesticated Artemisia absinthium population (Teruel, Spain), in comparison with a population (Sierra Nevada, Spain) undergoing the domestication process. Hydrolates from three crops of these two populations showed strong in vitro nematicidal effects against the root knot nematode Meloidogyne javanica and similar chemical profiles. The hydrolate of the A. absinthium population from Teruel strongly suppressed nematode egg hatching (>95%) after 5 days of incubation. In vivo tests on tomato seedlings showed a significant reduction in the of root penetration rate of M. javanica J2 treated with a hydrolate sublethal concentration (33%). In pot experiments, the hydrolate of A. absinthium tested on tomato plants significantly affected the nematode population, the infection frequency and the reproduction rate. The extraction of the organic fraction of the hydrolate gave a nematicidal extract and an inactive aqueous residue. The chemical profile of the active organic extract showed the presence of (5Z)-2,6-dimethylocta-5,7-diene-2,3-diol, previously described in the hydrodistilled essential oil of this A. absinthium population, among other compounds. This study demonstrates that the organic fraction of the A. absinthium hydrolate is a potential root-nematode control agent. Therefore, the hydrolate byproduct of the semi industrial vapor-pressure essential oil extraction could be a source of new nematicidal products.

Julio, Luis F.; González-Coloma, Azucena; Burillo Alquézar, Jesús; Díaz, Carmen E.; Andrés, Mª Fé

Crop Protection 94: 33-37 (2017)
Image
Biocidal effects of Piper hispidinervum

Biocidal effects of Piper hispidinervum (Piperaceae) essential oil and synergism among its main components

In this study we evaluated the effect of a pressure gradient (1–2 atm) in the extraction and composition of the essential oil (EO) of Piper hispidinervum by steam distillation. We also evaluated the insect antifeedant effects (Spodoptera littoralis, Leptinotarsa decemlineata, Myzus persicae and Rhopalosiphum padi) and nematicidal activity (Meloidogyne javanica) of the oils, their major components and their synergistic interactions. Safrole was the major component (78–81%) followed by terpinolene (5–9%). The EOs tested were effective insect antifeedants. Safrole, explained most of the insect antifeedant action of P. hispidinervum EOs. When safrole and terpinolene were tested in binary combinations, low ratios of safrole improved the antifeedant effects of terpinolene. P. hispidinervum EOs caused higher mortality of M. javanica juveniles than their major components. In binary combinations, low ratios of terpinolene increased the nematicidal effects of safrole. The EO treatment strongly suppressed nematode egg hatching and juvenile infectivity. P. hispidinervum EOs affected the germination of S. lycopersicum and L. sativa mostly at 24 h of treatment, being L. sativa the most sensitive. Safrole moderately affected germination and root growth of L. sativa, S. lycopersicum and L. perenne. Terpinolene only affected S. lycopersicum root growth.

Andrés, Mª Fé; Rossa, G. E.; Cassel, E.; Vargas, R. M. F.; Santana, O.; Díaz, Carmen E.; González-Coloma, Azucena

Food and Chemical Toxicology 109(2): 1086-1092 (2017)
Image
Chemical and biocidal characterization of two cultivated Artemisia absinthium

Chemical and biocidal characterization of two cultivated Artemisia absinthium populations with different domestication levels

The objective of this study was the characterization and valorization of the essential oil from a domesticated Artemisia absinthium population (Teruel, Spain), and its comparison with another one (Sierra Nevada, Spain) undergoing the domestication process. These populations are being experimentally cultivated in the same field since 2008. We studied their biomass and essential oil production (Clevenger hydrodistillation, HD and semi-industrial vapor-pressure, VP). The domesticated population showed lower chemical variation and higher biomass and essential oil yields, allowing for the registration of a new plant variety. The observed variations in oil composition (HD, VP) between the two populations were mostly quantitative. The oils were characterized by the presence of cis-epoxyocimene, (-)-. cis-chrysanthenol, chrysanthenyl acetate, linalool and trans-caryophyllene. The insect antifeedant (. Leptinotarsa decemlineata, Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi) and antifungal (. Fusarium spp. and Botrytis cinerea) effects of their oils were also tested. All VP extracts showed strong antifungal effects and the active antifungal compounds have been identified.

Julio, Luis F.; Burillo Alquézar, Jesús; Giménez, C.; Cabrera, Raimundo; Díaz, Carmen E.; Sanz Perucha, Jesús; González-Coloma, Azucena 

Industrial Crops and Products 76(15): 787-792 (2015)

Colaboraciones

Ricardo Guillermo Álvarez 

Departamento de Química Orgánica

Universidad de La Laguna

E-mail: riguial [at] ull.es