Esta sección incluye una lista de los últimos artículos científicos del IPNA publicados en revistas incluidas en el Science Citation Index (SCI).
En DIGITAL.CSIC, repositorio institucional del CSIC, pueden encontrar el listado completo de artículos científicos desde 1962, así como otras colecciones de interés como congresos, tesis, libros, material divulgativo, etc. del centro. El objetivo de DIGITAL.CSIC es organizar, preservar y difundir en acceso abierto los resultados de nuestra investigación.
En el repositorio institucional del CSIC, pueden encontrar el listado completo de artículos científicos, así como otras colecciones de interés como congresos, tesis, libros, material divulgativo, etc.
Análisis de la Producción Científica del IPNA 2014-2019: análisis bibliométrico realizado a partir de datos recogidos en Scopus y Web of Science.
Mapping stressed wheat plants by soil aluminum efect using C‑band SAR images: implications for plant growth and grain quality
Under toxic aluminum (Al) levels in the soil, wheat (Triticum aestivum L.) sufers stress and plant growth is afected. A method for diagnosis of plants is proposed that includes the following as a strategy: to analyze total Al in the soil, employ satellite radar imagery and calculate a vegetation index. The objective of this research, conducted at the feld scale, was to explore how radar backscattering coefcients from a winter wheat canopy, combined with the normalized diference vegetation index (NDVI) and geographic information system (GIS) technology, can be used as a mapping tool for the variability of Al-stressed canopies. As a result, an analysis of covariance showed signifcant diferences, and the lowest plant height was obtained at a high level of soil Al, as well as the minimum grain weight and magnesium content. It was found that a simple model could be used to estimate plant height from the backscattering coefcient of vertical transmit-vertical receive polarization (σ°VV), with a strong correlation (r−0.84). In turn, a third-order polynomial regression model (R20.70) was proposed to estimate the NDVI from σ°VV. This model provided a good estimate of the NDVI at the stem elongation stage of growth (50 days after sowing). Detected NDVI patterns were associated with variation in canopy stress depending on polarimetric information, which, in turn, was related to soil Al levels. Thus, the maps derived from the model can monitor spatial variability, where NDVI values<0.68 indicate stressed areas. This study provides guidance for in-season stress spatial variability caused by Al.
Hernández, Mercedes; Borges, Andrés A.; Francisco‑Bethencourt, Desiderio.
Cyclolauranes as plausible chemical scaffold against Naegleria fowleri
Primary amoebic meningoencephalitis (PAM) is a central nervous system (CNS) disease caused by Naegleria fowleri that mainly affects children and young adults with fatal consequences in most of the cases. Treatment protocols are based on the combination of different antimicrobial agents, nonetheless there is the need to develop new anti-Naegleria compounds with low toxicity and full effects compared to the currently used drug combination. The marine environment is a well-established source of bioactive natural products. In this work, we have focused on the structure of Laurencia cyclolaurane-type sesquiterpenes as potential chemical model against Naegleria species. The effects of debromolaurinterol (1) to induce PCD/apoptosis-like events in Naegleria fowleri have been evaluated, revealing that this compound induced reduction of ATP production showing a decrease of 99.98% in treated parasite cells. A SAR analysis have been supported with molecular modeling and analysis of the in silico ADME/Tox properties of the Laurencia sesquiterpenes debromolaurinterol (1), laurinterol (2) and allolaurinterol (3), which reinforce cyclolaurane metabolites as plausible molecular models to develop PAM treatments.
Arberas-Jiménez, Íñigo; García-Davis, Sara; Rizo-Liendo, Aitor; Sifaoui, Inés; Quintana Morales, Ezequiel; Piñero, José E.; Lorenzo-Morales, Jacob; Díaz-Marreno, Ana R.; Fernández, José J.
Applying Biostimulants to Combat Water Deficit in Crop Plants: Research and Debate
Climate change has increased the severity of drought episodes by further reducing precipitation in vulnerable zones. Drought induces a substantial decrease in agricultural water, reducing crop yields. Consequently, addressing water consumption can increase farmers’ profits. This work describes lab-to-field research in Zea mays, using two biostimulants: glycine betaine (GB) and Lpyroglutamic acid (PG). The biostimulant optimal dosages were selected using a hydroponic system with 20% polyethylene glycol and nursery experiments under water-deficit irrigation. The established dosages were evaluated in field trials in which irrigation was reduced by 20%. Laboratory biostimulant optimisation showed in stressed treated seedlings (GB 0.1 mM; PG 1 mM) an increased dry weight, relative growth rate and water use efficiency, reducing seedling growth loss between 65 and 85%, respectively. Field trials using a GB-optimised dosage showed an increase in plants’ growth, grain yield and flour Ca content. In addition, grain flour carbohydrate content and protein remained similar to control well-watered plants. Finally, the economic aspects of biostimulant treatments, water consumption, water sources (ground vs. desalinated) and grain biomass were addressed. Overall, GB treatment demonstrated to be a valuable tool to reduce water consumption and improve farmers’ earnings.
Jiménez-Arias, David; Hernándiz, Alba E.; Morales-Sierra, Sarai; García-García, Ana L.; García-Machado, Francisco J.; Luis, Juan C.; Borges, Andrés A.
Exploring the Agricultural Reutilisation of Desalination Reject Brine from Reverse Osmosis Technology
Among its other effects, climate change is driving humanity into water scarcity, especially in highly populated areas such as the Mediterranean. One of the best options to solve this problem is seawater desalination, but it has its environmental, social and economic impacts. The brine reject effluent from the process can be used as a rich mineral source for hydroponic culture. Indeed, stability in its composition was shown here during a one-year study. A compilation of analyses from desalination facilities located worldwide also demonstrates how reutilisation of this liquid waste can provide an opportunity to build a circular economy based on brine management. Potential profitable crops and an economic study of its implementation are discussed. Correct brine management channelled into an agricultural context can be an excellent way to improve the public and ecological reputation of desalination. It can also yield extra income beyond the fresh water yield from the proposed system.
Jiménez-Arias, David; Morales Sierra, Sarai; García-Machado, Francisco J.; García-García, Ana L.; Borges, Andrés A. ; Luis, Juan C.
Synthesis, Biological Evaluation, and Molecular Docking Study of 3-Amino and 3-Hydroxy-seco A Derivatives of α-Amyrin and 3-Epilupeol as Inhibitors of COX-2 Activity and NF-kB Activation
In this study, a series of novel 3-seco-A derivatives of the natural triterpenes α-amyrin (1) and 3-epilupeol (2) were synthesized by a one-pot radical scission–oxidation procedure and evaluated in vitro and in vivo for their capacity to inhibit the inflammatory process. For the in vitro studies, the trans-4-hydroxy-l-proline methyl ester derivatives (1f and 2f) were consistently effective in inhibiting NO, IL-6, and TNF-α secretion, as well as inhibition of NF-κB activation, in RAW cells stimulated by LPS. The further in vivo anti-inflammatory study revealed that the trans-4-hydroxy-l-proline methyl ester derivatives (1f and 2f), together with 1g, were the most effective in inhibiting TPA-induced edema. Interestingly, the α-amyrin derivatives were the most potent inhibitors of COX-2, but inhibited COX-1 only to some extent. The hydroxyl derivative (1c) was selective for COX-2 inhibition (66.3 ± 1.1% at 17.5 μM) without affecting the COX-1 isoform and did not present toxicity. Molecular docking studies revealed that these compounds bind with their polar region in the cavity over Arg-120, and their lipophilic part is orientated to the HEM cofactor similarly to the natural substrate arachidonic acid in the catalytic site of COX-2. These results indicated that seco-A ursane derivatives could be considered promising candidates for the future development of selective NF-κB and COX-2 inhibitors.
Romero-Estrada, Antonio; Boto, Alicia; González-Christen, Judith; Romero-Estudillo, Iván; Garduño-Ramírez, María Luisa; Said Razo-Hernández, Rodrigo; Marquina, Silvia; Maldonado-Magaña, Amalia; Columba-Palomares, María C.; Sánchez-Carranza, Jessica Nayelli; Álvarez, Laura.
Impact of Volcanic Sulfur Emissions on the Pine Forest of La Palma, Spain
In autumn 2021, the largest volcanic eruption on the island of La Palma in historic records took place. The Canary Islands are of volcanic origin and eruptions have always constituted part of their natural disturbance regime. Until recently, their impacts could not be directly observed and studied. Influence of the emission of phytotoxic gases on biodiversity and ecosystem dynamics was hitherto unknown. The recent eruption is still being intensely monitored. We used Sentinel-2 remote sensing data to analyze the spatial extent and intensity of the impact related to sulfuric emissions, aiming to understand the damage patterns in Canary pine forest. The emissions damaged 10% of that forest and affected 5.3% of the Natura 2000 protected areas. We concluded that this is largely due to the toxic effects of the enormous emissions of SO2. We found a clear correlation between the change in the normalized difference vegetation index (NDVI) and distance from the eruption. This pattern was weakly anisotropic, with stronger damage in southern directions. Counteracting effects, such as ash deposition, were largely excluded by combining NDVI change detection with tree cover density. We expect that vegetation damage will be transient. P. canariensis can resprout after forest fires, where most leaves are lost. Consequently, our assessment can serve as a reference for future ecosystem regeneration.
Wiser, Frank; Baumann, Esther; Jentsch, Anke; Medina, Félix Manuel; Meng, Lu; Nogales, Manuel; Beierkuhnlein, Carl.
Sustainable Production of Insecticidal Compounds from Persea indica
In this work, we have investigated the accumulation of ryanoids in different plant parts (leaves, stems and roots) of aeroponically grown Persea indica cloned trees (one-year-old cloned individuals) and a selected mature, wild tree. We tested the insect antifeedant (against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi) and nematicidal (against Meloidogyne javanica) effects of ethanolic extracts from these different plant parts. The HPLC-MS analysis of P. indica extracts showed that mature tree (wild) leaves had two times more chemical diversity than stems. Aeroponic plants showed fewer differences in chemical diversity between leaves and stems, with the lowest diversity found in the roots. Ryanodane epiryanodol (1) was present in all the plant parts, with the mature stems (wild) containing the highest amount. The aeroponic stems also accumulated ryanoids including 1, cinnzeylanol (2) and cinnzeylanone (4). The insect Spodoptera littoralis was strongly affected by the stem extracts, while leaf extracts were moderately active. Based on predicted vs. real antifeedant values, we concluded that the ryanoid content (1 or a combination of 2, 4 and 1) explained the antifeedant effects of the stem extracts, while additional components contributed to the activity of the leaf extracts. Therefore, careful individual selection of P. indica seedlings should be carried out prior to proceeding with aeroponic cultivation in order to obtain ryanodane-rich stem or leaf extracts with strong antifeedant effects on S. littoralis.
González-Coloma, Azucena; Andrés, María Fe; Contreras, Rodrigo; Zúñiga, Gustavo E.; Díaz, Carmen E.
The Nitration of Proteins, Lipids and DNA by Peroxynitrite Derivatives-Chemistry Involved and Biological Relevance
In recent years, much interest has been generated by the idea that nitrosative stress plays a role in the aetiology of human diseases, such as atherosclerosis, inflammation, cancer, and neurological diseases. The chemical changes mediated by reactive nitrogen species (RNS) are detrimental to cell function, because they can cause nitration, which can alter the structures of cellular proteins, DNA, and lipids, and hence, impair their normal function. One of the most potent biological nitrosative agents is peroxynitrite (ONOO¯), which is produced when nitric oxide (*NO) and superoxide (*O2¯) are combined at extremely rapid rates. Considering the plethora of oxidations by peroxynitrite, this makes peroxynitrite the most prevalent nitrating species responsible for protein, DNA, and lipids nitration in vivo. There is biochemical evidence to suggest that the interactions of the radicals NO and superoxide result in the formation of a redox system, which includes the reactions of nitrosation and nitration, and is a component of the complex cellular signalling network. However, the chemistry involved in the nitration process with peroxynitrite derivatives is poorly understood, particularly for biological molecules, such as DNA, proteins, and lipids. Here, we review the processes involved in the nitration of biomolecules, and provide a mechanistic explanation for the chemical reactions of NOS and nitrosative stress. This study reveals that these processes are based on a surprisingly simple and straightforward chemistry, with a fascinating influence on cellular physiology and pathology.
Pérez de Lastra, José Manuel; Andrés Juan, Celia; Plou Gasca, Francisco José; Pérez-Lebeña, Eduardo.
Free-Radical Epimerization of D- into L-C-(glycosyl)methanol Compounds Using 1,5-Hydrogen Atom Transfer Reaction
A simple epimerization of C-(α-D-Gly p )methanol into C-(β-L-Gly p )methanol compounds is described. The radical sequence involved homolytic cleavage of the C5–H bond by 1,5-hydrogen atom transfer promoted by the 1’-O-yl radical and subsequent hydride addition with inversion of configuration. This methodology allows the preparation of rare C-(β-L-Ido p )-, C-(β-L-Altp)-, C-(β-L-Gulp)-, C-(β-L-Allp)methanol glycosides starting from carbohydrates of the D-series. It can also be applied to transform L- into D-configured C-glycosyl compounds, as illustrated by the formation of C-(β-D-6dGulp)- and C-(β-D-6dAltp)methanol from readily accessible L-rhamno and L-fuco analogs, respectively. In further development of this procedure compounds with C-(β-L-Araf)- and C-(β-L-5dRibf)methanol structures have also been synthesized. The alkoxyl radicals were generated by reaction of the corresponding N-alkoxyphthalimides with nBu3SnH(D) and in comparative terms, by visible light-photocatalysis using the Hantzsch ester/fac-Ir(ppy)3 procedure. The influence of the sugar ring conformation and the electronegativity of the substituents on the stereochemical outcome will be addressed.
Montes, Adrián S.; León, Elisa I.; Martín, Ángeles; Pérez-Martín, Inés; Suárez, Ernesto
Osteoprotective effect of the marine alkaloid norzoanthamine on an osteoporosis model in ovariectomized rat
Norzoanthamine (NZ), an alkaloid that has been isolated from the marine cnidiaria Zoanthus sp., has been shown an interesting anti-osteoporotic activity. Although its mechanism of action is not yet clear, it seems that it is different from those of currently used drugs making it particularly interesting. Previous studies have been carried out mostly in vitro. Herein, we present an in vivo study that allows to check the real potential of NZ as a protector substance by direct application into ovariectomized rat bone using a sustained delivery system. Histological and histomorphometric results in ovariectomized rats showed higher bone quality as a result of greater number of trabeculae and osteogenic activity in the group implanted with NZ, compared to controls. In contrast with the untreated controls, NZ-treated groups showed a balanced osteoblast/osteoclast number ratio, similar to that found in the normal bone. These results suggest that NZ could be useful as adjunct to other osteoporosis treatments, but probably its main therapeutic role would be as preventive therapy against bone deterioration.
García-García, Patricia; Reyes, Ricardo; Évora, Carmen; Delgado, Araceli; Fernández, José J.; Hernández Daranas, Antonio.