Publicaciones

Esta sección incluye una lista de los últimos artículos científicos del IPNA publicados en revistas incluidas en el Science Citation Index (SCI).

En DIGITAL.CSIC, repositorio institucional del CSIC, pueden encontrar el listado completo de artículos científicos desde 1962, así como otras colecciones de interés como congresos, tesis, libros, material divulgativo, etc. del centro. El objetivo de DIGITAL.CSIC es organizar, preservar y difundir en acceso abierto los resultados de nuestra investigación.

En el repositorio institucional del CSIC, pueden encontrar el listado completo de artículos científicos, así como otras colecciones de interés como congresos, tesis, libros, material divulgativo, etc.

Ir a Digital - CSIC

 

Análisis de la Producción Científica del IPNA 2014-2019: análisis bibliométrico realizado a partir de datos recogidos en Scopus y Web of Science.

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Digital CSIC

Combining in-situ monitoring and remote sensing to detect spatial patterns of volcanic sulphur impact on pine needles

Volcanic eruptions have a strong environmental impact on surrounding forests. Trees are affected by mechanical damage, tephra deposition and volcanic gases. Oceanic islands are shaped by relatively frequent volcanic eruptions and thus offer the opportunity to study the effect of volcanic activity on biodiversity. We investigate the impact of volcanic gas emissions and tephra deposition during the 2021 Tajogaite eruption on the Canary Pine forests of the island of La Palma, Spain, characterized by monospecific stands of the endemic pine species Pinus canariensis C. Sm. ex D.C. Large quantities of volcanic sulphur dioxide caused chlorotic damage up to approximately 7 km around the crater, followed by widespread resprouting of P. canariensis. To detect the spatial pattern of impacts, we sampled P. canariensis needles from all over the island of La Palma and analyzed their sulphur (S), nitrogen (N) and carbon (C) content. We found a strong increase of S needle content close to the crater, while C decreased significantly. S levels were strongly related to distance to the crater, C and N were mostly influenced by S content. Trees affected by volcanic gases allocate resources to resprouting, leading to lower levels of C due to translocation of C as a building block. Surprisingly, we found higher N levels in needles with high levels of S and a less clear pattern compared to C, likely due to a multitude of environmental factors influencing N needle levels. We investigated how canopy damage patterns detected in Sentinel-2 remote sensing imagery after the eruption correlated to the in-situ needle contents. However, we did not find a clear correlation between in-situ needle values and spectral responses in remote sensing. While satellite images were well suited to analyse large scale patterns of canopy damage following the eruption, needle levels varied strongly on a local, tree-based level, which is not reflected in remote sensing imagery.

Weiser, Frank; Walentowitz, Anna; Baumann, Esther; Shatto, Christopher; Guerrero-Campos, María; Jentsch, Anke; Nogales, Manuel; Medina, Félix Manuel; Vetaas, Ole Reidar; Beierkuhnlein, Carl.

Forest Ecology and Management, 549(1), 121468: 1-17 (2023)
DOIDigital.CSIC

Conformational control enables boroxine-to-boronate cage metamorphosis

The discovery of molecular organic cages (MOCs) is inhibited by the limited organic-chemical space of the building blocks designed to fulfill strict geometric requirements for efficient assembly. Using intramolecular attractive or repulsive non-covalent interactions to control the conformation of flexible systems can effectively augment the variety of building blocks, ultimately facilitating the exploration of new MOCs. In this study, we introduce a set of boronic acid tripods that were designed using rational design principles. Conformational control was induced by extending the tripod's arms by a 2,3-dimethylbenzene unit, leading to the efficient formation of a tetrapodal nanometer-sized boroxine cage. The new building block's versatility was demonstrated by performing cage metamorphosis upon adding an aromatic tetraol. This led to a quantitative boroxine-to-boronate transformation and a topological shift from tetrahedral to trigonal bipyramidal.

Rondelli, Manuel; Delgado-Hernández, Samuel; Hernández Daranas, Antonio; Martín, Tomás.

Chemical Science: 1-8 (2023)
DOIDigital.CSIC

Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential

This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.

Mehmood, Ansar; Zahir, Sobia; Khan, Muhammad Abdul Rauf; Ahmad, Khawaja Shafique; Abasi, Fozia; Raffi, Muhammad; Proćków, Jarosław; Pérez de Lastra, José Manuel.

Journal of Biomolecular Structure and Dynamics: 1-11 (2023)
DOIDigital.CSIC

Induction of New Aromatic Polyketides from the Marine Actinobacterium Streptomyces griseorubiginosus through an OSMAC Approach

Using the OSMAC (One Strain Many Compounds) approach, the actinobacterium Streptomyces griseorubiginosus, derived from an unidentified cnidarian collected from a reef near Pointe de Bellevue in Réunion Island (France), was subjected to cultivation under diverse conditions. This endeavour yielded the isolation of a repertoire of 23 secondary metabolites (1–23), wherein five compounds were unprecedented as natural products (19–23). Specifically, compounds 19 and 20 showcased novel anthrone backbones, while compound 23 displayed a distinctive tetralone structure. Additionally, compounds 21 and 22 presented an unusual naphtho [2,3-c]furan-4(9H)-one chromophore. Interestingly, the detection of all these novel compounds (19–23) was exclusively achieved when the bacterium was cultured in FA-1 liquid medium supplemented with the epigenetic modifier γ-butyrolactone. The elucidation of the structural features of the newfound compounds was accomplished through a combination of HRESIMS, 1D and 2D NMR spectroscopy, as well as QM-NMR (Quantum Mechanical—Nuclear Magnetic Resonance) methods and by comparison with existing literature. Moreover, the determination of the relative configuration of compound 23 was facilitated by employing the mix-J-DP4 computational approach.

Rodríguez Martín-Aragón, Víctor; Romero Millán, Francisco; Cuadrado, Cristina; Hernández Daranas, Antonio; Fernández Medarde, Antonio; Sánchez López, José M.

Marine Drugs 21(10): 526 (2023)
DOIDigital.CSIC

La imperiosa necesidad de programar la asistencia a urgencias, atendiendo al cambio climático y las crisis de calidad del aire

El cambio climático y la crisis global de calidad del aire son fenómenos que están teniendo impactos en numerosos aspectos de la sociedad, incluyendo la atención médica en general y en los servicios de urgencias en particular. En 2021 la Organización Mundial de la Salud declaró que el cambio climático es “la mayor amenaza para la salud a la que enfrenta la humanidad”1, motivo por el que este organismo publicó nuevas directrices de calidad del aire, recomendando reducir drásticamente los niveles de contaminación en el aire ambiente. Recientemente, y en relación a los escenarios meteorológicos que estamos viviendo en este nuevo escenario climático, el secretario general de la ONU declaro “la humanidad ha abierto las puertas del infierno”.

Benito Lozano, Miguel; Rodríguez, Sergio.

Revista Española de Urgencias y Emergencias, 2: 194-197 (2023)
Digital.CSIC

TBS-pyrrole as an “universal” reference to quantify artemisinin and structurally-diverse natural products in plants extracts by NMR

The commercial production of artemisinin and other valuable bioactive natural products depends on their plant sources, which may provide variable amounts of the compound depending on plant variety, the period of the year, abiotic stress and other factors. Therefore, it requires a method for large-scale, low-cost natural product quantification. The standard HPLC and UHPLC methods are accurate but the analysis are costly and require different optimization for structurally-diverse products. An alternative method using NMR with TBS-pyrrole as a novel “universal” reference affords a simple, fast method to quantify many different products. The method is shown with antimalarial artemisinin, whose yield using conventional and novel extraction procedures was determined by standard UHPLC-MS procedures and by our NMR protocol, with similar quantification results. The novel reference compound does not interfere with artemisinin or extract signals, only needs a small amount of the extract, is accurate and operationally simple, and a large volume of samples can be processed in little time. Moreover, bioactive terpenes, steroids, alkaloids, aromatic compounds, and quinones, among others, were quantified in a model vegetal extract with this “universal” reference with excellent accuracy.

García-García, Ana L.; Hernández, Dácil C; Santana-Mayor, Álvaro; Jiménez-Arias, David; Boto, Alicia.

Frontiers in Plant Science 14, 1255512 : 1-15 (2023)
DOIDigital.CSIC

Síntesis de éteres cíclicos naturales

Se presenta un resumen de algunos métodos utilizados en la síntesis de productos naturales bioactivos de origen marino. El artículo se estructura acorde al orden en que son introducidos los enlaces más importantes en los heterociclos oxigenados. Se discute asimismo la similitud de algunos métodos sintéticos utilizados con los usados por los organismos vivos productores.

Betancort, Juan M.; Carrillo Fumero, Romen; Díaz Díaz, David; García, Celina; Martín, Tomás; Padrón, Juan I.; Padrón, José M.; Ramírez, Miguel Á.; Martín, Víctor S.

Anales de química, 119(3): 153-160 (2023)
Digital.CSIC

First report of Aphria latifrons (Diptera, Tachinidae, Leskiini) in the Canary Islands

The Canary Islands are an archipelago of volcanic origin, located off north-west Africa comprising eight islands. Fuerteventura and Lanzarote are the oldest (20 and 15 millon years old, respectively) and the easternmost islands. The order Diptera is one of the most relevant taxa in the Canary Islands as they constitute the second highest species richness. Within this order, the family Tachinidae is especially interesting as all species are endoparasitoids of arthropods and most species play a key role as pollinators. In the Canary Islands, the family comprises 52 species, with Fuerteventura and Lanzarote harbouring up to 20 species each.

Suárez, Daniel; Lugo, David; Pérez-Gil, Mónica; Peña, Gustavo; Ruiz, Carlos.

Biodiversity Data Journal, 11, e109690: 1-9 (2023)
DOIDigital.CSIC

Geography, climate and shifts in host plants distribution explain the genomic variation in the cactus moth

Landscape heterogeneity and the host plant use are factors suggested to play determinant roles in shaping the evolutionary history of herbivorous insects. However, the role of the reconfiguration of host plants distributions linked to Quaternary climate oscillations as drivers of contemporary population genetic structure is still poorly understood. Here, we formally examine the relative contribution of such factors on intraspecific diversification using the South American cactus moth, Cactoblastis cactorum, an herbivore insect specialized in the use of cacti as host plants. We assessed genomic variation using genome-wide SNPs and mitochondrial data in populations sampled across a broad geographical gradient where moths feed on different cactus species. We integrated demographic simulations and ecological niche modeling into a landscape genomics framework, to test alternative hypotheses of past and current population connectivity for both C. cactorum and its host plants. Regions exhibiting higher genomic diversity were evaluated for congruence with areas where suitable climatic conditions remained stable through time. Our results revealed that past spatial configuration of suitable habitat conditions and shifts of host plants distributions are the factors that better explain the intraspecific diversification. Genomic data also supported the hypothesis that areas of long-term habitat stability served as refugia for C. cactorum, enabling the maintenance of high levels of genetic diversity over time. Overall, our study highlights the importance of integrating inter-specific interactions and their spatio-temporal dynamics to better understand the relative importance of abiotic and biotic factors driving the diversification processes in herbivorous insects with broad geographical and restricted host ranges.

Poveda-Martínez, Daniel; Noguerales, Víctor; Hight, Stephen D.; Logarzo, Guillermo; Emerson, Brent C.; Varone, Laura; Hasson, Esteban.

Frontiers in Ecology and Evolution, 11: 1-14 (2023)
DOI

Ligand-Directed Chemistry on Glycoside Hydrolases – A Proof of Concept Study

Selective covalent labelling of enzymes using small molecule probes has advanced the scopes of protein profiling. The covalent bond formation to a specific target is the key step of activity-based protein profiling (ABPP), a method which has become an indispensable tool for measuring enzyme activity in complex matrices. With respect to carbohydrate processing enzymes, strategies for ABPP so far involve labelling the active site of the enzyme, which results in permanent loss of activity. Here, we report in a proof of concept study the use of ligand-directed chemistry (LDC) for labelling glycoside hydrolases near – but not in – the active site. During the labelling process, the competitive inhibitor is cleaved from the probe, departs the active site and the enzyme maintains its catalytic activity. To this end, we designed a building block synthetic concept for small molecule probes containing iminosugar-based reversible inhibitors for labelling of two model β-glucosidases. The results indicate that the LDC approach can be adaptable for covalent proximity labelling of glycoside hydrolases.

Prasch, Herwig; Wolfsgruber, Andreas; Thonhofer, Martin; Culum, André; Mandl, Christoph; Weber, Patrick; Zündel, Melanie; Nasseri, Seyed A.; González Santana, Andrés; Tegl, Gregor; Nidetzky, Bernd; Gruber, Karl; Stütz, Arnold E.; Withers, Stephen G.; Wrodnigg, Tanja M.

ChemBioChem, e202300480: 1-12 (2023)
DOIDigital.CSIC